anti-oxidant and anti-inflammatory cyclic diarylheptanoids from alnus japonica stem bark

a new cyclic diarylheptanoid namely alnuheptanoid b (3), along with four known cyclic diarylheptanoids: myricanone (1), (+)-s-myricanol (2), myricanone 5-o--d-glucopyranoside (4), and (+)-s-myricanol 5-o--d-glucopyranoside (5) were isolated from the etoac fraction of alnus japonica steud (family: betulaceae) stem bark. their structures were established by different spectroscopic analyses, as well as optical rotation measurement. compounds 1, 2, 4, and 5 are isolated for the first time from a. japonica. the antioxidant and anti-inflammatory activities of compounds (1-5) were assessed using dpph assay and carrageenin induced rat paw edema model, respectively. they displayed significant antioxidant activity in relation to propyl gallate (standard antioxidant) at concentration 50 µm. compound 2 demonstrated anti-inflammatory effect at a dose 10 mg/kg compared with indomethacin (positive control).